Electroporation is the most effective non-viral technique for exogenous gene transfer, and it has been applied extensively due to its convenience and high efficiency.

电穿孔基因转移技术是外源基因转移最为有效的一种非病毒载体法，其突出的优点是简便、高效，已广泛用于体外基因转移。

Therefore, applications of the electroporation transdermal drug delivery technology in clinical has great potential and good prospect.

因此将电致孔经皮给药技术应用于临床,具有巨大的潜力与良好的应用前景。

Methods A signal peptide sequence(sp) was synthesized and cloned into the integrative plasmid pIlac down-stream of lactose-inducible promoter. The recombined plasmid pIlac-sp-IL10 was then introduced into Lactobacillus casei(L. casei)CECT 5276 by electroporation.

方法人工合成信号肽系列后与IL-10基因连接后克隆到干酪乳酸杆菌(Lactobacillus casei,L.casei)整合型表达载体pIlac的lac启动子下游,得到重组质粒pIlac-sp-IL10,电穿孔转化L.caseiCECT 5276。

Therefore maintaining the osmotic pressure may be an effective way to improve the rate of electroporation.

在电击转化过程中,维持细胞渗透压是提高转化效率的有效方法。

The result of sequencing showed that the insert gene could encode SOD correctly; After successfully transforming pMG36e-sod into L6032 by electroporation the activity of SOD was about 590.5NU∕mg prot.

核酸测序显示插入的sodA片段能正确编码SOD氨基酸序列。 利用电转化成功地将重组质粒导入L6032中,SOD的表达活性约590.5NU/mgprot。

The results showed that electroporation can not bring on an obvious enhancement of transformation efficiency.

以期能够获得高效转化最佳条件,结果发现电击转化并不能成倍提高转化效率。

To simplify the approach, we replaced the electroporation method by heat-shock method for E. coli transformation and eliminated the procedures as dialysis etc.

同时，用热激法替代了电击法进行质粒的大肠杆菌转化，并去掉了透析等步骤，简化了构建过程。

Major methods for genetransformation include Agrobacterium mediated, PEG untake, electroporation,particle gun, microinjection, laser microbeam, pollen-tube pathway, ect. . Genetransformation mediated by Agrobacterium is widely used in Brassica breeding.

基因工程技术主要包括农杆菌介导转化法，PEG原生质体融合法，电激法，基因枪法，显微注射法，花粉管通道法等。