In this experiment Codetta (♀)and Dorper (♂)sheep breeding hybrid muscle total RNA as a template, cDNA was synthesized by reverse transcription. According to the known sheep MSTN cDNA sequence, designed five-specific primers used nested PCR, MSTN gene was amplified.
本实验以小尾寒羊和杜泊羊的杂交种羊骨骼肌总RNA为模板,反转录合成cDNA,根据已知的绵羊MSTN基因cDNA序列,设计了5条特异性引物,采用巢式PCR,扩增得到MSTN基因。
参考来源 - 绵羊Myostatin基因cDNA全序列的克隆及在CHO中的表达