Overexpression of the C-terminal domain of Tob1a, which lacks N-terminal BoxA and BoxB, leads to dorsalization of the injected embryos, slight increase of the embryonic shield marker goosecoid, and decrease of the ventral marker eve1. It suggests that Tob1a plays a role in regulating dorsal-ventral patterning of zebrafish embryos.
在斑马鱼胚胎中过量表达Tob1a的C－端区域，24小时胚胎出现背部化特征，早期胚胎中胚盾标记基因goosecoid的表达略有增强，而腹部标记基因eve1的表达下降，表明Tob1a可能在胚胎的背腹分化中起作用。
参考来源 - 斑马鱼两个Tob家族基因的研究