The fermentation conditions for alcohol production using immobilized beads were: 4% sodium alginate, 0.9% chitosan, fermentation temperature 30-35℃, and with a immobilization usage of 0.25 g/ml. Saccharomyces cerevisiae immobilized by chitosan-alginate beads were used to ferment beet in bioreactors, the immobilized Saccharomyces cerevisiae could maintain fermentability for a long time and could be used continuously, which would be beneficial in the continuous production in industry.

壳聚糖-海藻酸钠固定化小球进行酒精发酵时的单因素发酵条件为:海藻酸钠浓度为4%,最适温度在30℃～35℃之间,填充量选择0.25g/mL,壳聚糖浓度最适量可以选择0.9%,这样的发酵效果最好,酒精转化率最高。