noncoaxial refold非共轴重褶
purify and refold纯化和复性
How to refold inclusion body proteins with many pairs of disulfide bonds into natural conformation with high efficiency is a challengeable problem at present.
如何对富含二硫键的包涵体蛋白进行高效的体外复性是重组蛋白生产所面临的巨大挑战。
参考来源 - 折叠酶DsbA的制备及协助蛋白质体外复性的研究We refold the protein through quick dilution. After desalt,we purified the protein by the affinity resin.
采用快速稀释方式进行蛋白复性，采用脱盐柱脱盐后，采用亲和层析纯化制备高纯度的蛋白。
参考来源 - 抗肝癌重组单链Fv段—免疫毒素（scFv25