Oxidative renaturation氧化复性
dna renaturationDNA复性;复性
renaturation condition复性条件
renaturation yield复性率
Renaturation DNA的复性;复性作用
renaturation solution复性液
complete renaturation完全复性
protein renaturation蛋白再生;蛋白复性
renaturation of蛋白质复性
IB solubilized in the presence of 8mol/L urea were diluted with renaturation solutions so that folding process initiated. Concentrated IL-3 were digested with thrombin and authentic rhIL-3 released.
超声破碎细菌细胞得包涵体,经洗涤处理可使包涵体纯度达90％,用8mol/L尿素变性溶解包涵体沉淀后直接稀释复性,再超滤浓缩、凝血酶消化,释放天然型rhIL-3。
参考来源 - 变性剂诱导的蛋白溶菌酶分子去折叠和重折叠过程各稳定构象态的分布和过渡The activity of expressed protein after renaturation had recovered and further confirmed by lymphocyte proliferation assay in vitro.
ELISA鉴定复性后的蛋白已恢复活性,并能在体外刺激外周血单个核细胞的增殖。
参考来源 - 人源鼻咽癌双价抗独特型抗体疫苗的制备及活性鉴定